The pipette tip is one of the essential tools in the laboratory. In the pursuit of precision and error-free experiments, the selection of different quality, model, and brand pipette tips may affect the results of the experiment. Choosing the appropriate injection molding machine for pipette tip is the first step to a successful experiment.

In the previous blog, we mainly introduced the specifications of pipette tips. This blog let us mainly focus on how to use pipette tips to ensure the accuracy of the experiment correctly.

How to operate pipette tip correctly?

Use the appropriate tip

To ensure better accuracy and precision, it is recommended that the pipetting volume be within the range of 35%-100% of the tip.

Installation of the suction head

For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the tip: in order to pursue a good seal, you need to insert the pipette handle into the tip, turn it left and right or shake it back and forth to tighten it forcefully. There are also people who use the pipette to repeatedly hit the tip to tighten it, but this operation will cause the tip to deform and affect the accuracy. In severe cases, the pipette will be damaged, so such operations should be avoided.

The immersion angle and depth of the tip

The immersion angle of the tip is controlled within 20 degrees of inclination, and it is better to keep it upright; the tip immersion depth is recommended as follows:

Pipette specification tip immersion depth:

2L and 10 L - 1 mm

20L and 100 L - 2-3 mm

200L and 1000 L - 3-6 mm

5000 L and 10 ML - 6-10 mm

Suction head rinse

For samples at room temperature, tip rinsing can help improve accuracy; but for samples with high or low temperature, tip rinsing will reduce the accuracy of the operation. Please pay special attention to users.

Liquid suction speed

The pipetting operation should maintain a smooth and appropriate suction speed; too fast aspiration speed will easily cause the sample to enter the sleeve, causing damage to the piston and seal ring and cross-contamination of the sample.

[some suggestion]

1. Maintain the correct posture when pipetting; do not hold the pipette tightly all the time, use a pipette with a finger hook to help relieve hand fatigue; change hands frequently if possible.

2. Regularly check the sealing condition of the pipette. Once it is found that the seal is aging or leaks, the sealing ring must be replaced in time.

3. Calibrate the pipette 1-2 times a year (depending on the frequency of use).

For most pipettes, before use and after a period of use, the piston should be coated with lubricating oil to maintain the seal; for pipettes of conventional volume, the pipette with no lubricating oil will also have an ideal seal.

The pipette tip is a consumable that is used in large quantities in the laboratory, so while maintaining mass production, it is also necessary to comply with the use specifications of the injection molding machine for pipette tip

Note before use:

• Check whether the solid nuts at each part are loose.

• Before starting the machine, the lubrication parts should be filled with lubricating oil. During the production process of the machine, it is necessary to check the lubrication condition of each part once every 2-3 hours, and add enough lubricating oil (grease) at any time.

• If it is found that the parts on the equipment are malfunctioning or damaged and can no longer start production, they should promptly report to the relevant personnel in the workshop, and they should not handle it by themselves, and make a record.

• For equipment that has been found to have problems, it is not allowed to start production and use without repairing and troubleshooting.

• Check the quality and quantity of hydraulic oil frequently. Keep the quantity of hydraulic oil within the display range of the oil level in the oil tank. When the hydraulic oil level is close to the low level of the oil level, add oil appropriately. The hydraulic oil should be replaced with new oil according to the oil quality when working for one year.

• Frequently detect changes in the temperature of the hydraulic oil when the hydraulic transmission system is working, and increase the flow of the hydraulic oil cooling water when the temperature of the hydraulic oil begins to rise.

• When the operator needs to leave the machine temporarily for some reason, the power should be cut off.

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